For FAST post-labeling purification of LudgerTag fluorophore and chromophore labeled glycans. Suitable for cleanup after glycan labeling with 2-AB (2-aminobenzamide) and 2-AA (2-aminobenzoic acid).Each cartridge can typically bind glycans released from up to 50-100 µg glycoprotein. Total procedure complete in 45 minutes when using our vacuum manifold system.
View Detailed Description
View product documentation as PDF
View Certificate of Analysis as PDF
contains 6 cartridges
Part Number: LC-T1-A6
One of the most reliable methods for achieving post-labeling cleanup is use of S-cartridges. These employ a hydrophilic membrane to separate the labeled glycans from non-glycan contaminants and are routinely used in the biopharma industry during glycoprofiling QC for lot release of FDA and EMA approved biopharmaceuticals.
View photo guide
Part Number: LC-S-A6
LudgerClean EB10 cartidges have been designed for purification of glycans from proteins, salts, and detergents.The cartridges contain a 10 mg bed of Ludger Electron Interaction Resin (EIR). This unique material contains very flat regions of pi-orbital electrons that interact with hydrophilic organic compounds including glycans.The resin can capture a wide range of glycans from complex mixtures while salts and certain detergents pass through. The glycans are then eluted from the cartridge using a simple solvent system.The cartridge housing is constructed of polypropylene and is compatible with many automated sample preparation systems.
Part Number: LC-EB10-A6
The LudgerClean™ PBM Post-Glycosidase Clean-Up plate (Cat #LC-PBM-96) is a 96-well plate system that has been developed for removal of endoglycosidase and exoglycosidase enzymes from 2-AA or 2-AB labelled N-glycans after glycan sequencing. It can also be used with unlabelled glycans.
This will prevent contamination of HPLC columns during subsequent chromatographic analysis. The plate can also be used to remove glycosidases or other proteins before mass spectrometry analysis of glycans.The LC-PBM-96 plate is a 96 well membrane-bottom plate containing a specialized protein binding membrane with a nominal pore size of 0.45 µm. The plate is designed for use with the Ludger-Velocity vacuum manifold or with other popular vacuum manifold systems to give excellent clean up of up to 96 samples simultaneously.
Part Number: LC-PBM-96
For removal of excess procainamide label after procainamide labeling of glycans. This clean-up plate is used where excess procainamide interferes with subsequent sample analysis.
The LC-PROC-96 plate is a 96 well membrane-bottom plate containing a specialized glycan binding membrane. This product is designed for use with both the vacuum manifold that can be purchased from Ludger or with other popular vacuum manifold systems. Excess procainamide label passes through the membrane whilst glycans are bound to the membrane allowing separation of these two components.
Part Number: LC-PROC-96
For purification of O-glycans from non-carbohydrate material including salts, proteins,and detergents. Applications include cleanup of glycans following hydrazinolysis or anyother non-reductive O-glycan release method
Each LudgerClean™ CEX cartridge can typically clean up O-glycans released from1000 µg of glycoprotein.
View Detailed Description
Part Number: LC-CEX-A6
The 96 well plate is used for removal of excess salts, detergent, proteins and other impurities after the release of N-glycans from glycoproteins. This clean-up plate is used to enrich N-glycans prior to performing permethylation of released glycans.
Part Number: LC-PERMET-96