Deglycosylation kits

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	Deglycosylation kits
		Enzymatic DeGlycoMx Kit
		Enzymatic CarboRelease Kit
		Orela O-linked Glycan Release Kit
		Endo F Multi-Kit
		PNGase F
		PNGase F - recombinant
	Endoglycosidases
	Exoglycosidases
	Sialic Acid Products
	Purification Cartridges and Plates
	Glycan Labeling Kits
	HPLC Columns and Buffers
	Glycan Standards
	CarboSequencing
	MS/MS Data Analysis Tools

Deglycosylation kits

QA-Bio provides easy to use kits for deglycosylating proteins in both native & denatured conditions.

PNGase F is commonly used to deglycosylate glycoproteins containing only N-linked glycans, working in both denatured and native conditions. For native samples that are slowly cleaved by PNGase F or those that precipitate after PNGase F digestion, our Endo F Multi-Kit is recommended. Both methods leave the glycans intact and available for further analysis.

If the glycan type is unknown, or if it consists of both N-linked & O-linked glycans, the use of our Enzymatic CarboRelease Kit or our Enzymatic DeGlycoMx Kit is recommended. These kits include a number of enzymes which will remove and digest the glycans into monosaccharides. In the DeglycoMx Kit, the enzymes are packaged as a premixed cocktail of enzymes in one 20 microlitre vial. For the CarboRelease Kit, the enzymes are supplied separately in individual vials of 20 microlitres, allowing greater flexibility in assay design.

For release and recovery of O-linked Glycans, we recommend our Ludger Orela kit.

Enzymatic DeGlycoMx Kit

KE-DGMX

This kit includes our DeGlycoMx, a premixed cocktail of the enzymes required to remove all N-linked oligosaccharides and most O-linked sugars from 0.5 mg of glycoprotein, via 10 reactions of up to 50 micrograms of protein per reaction.

The kit is easy-to-use and effective: add 2 µl of the DeGlycoMx enzyme to your denatured sample and the included buffer and incubate for 3 hours at 37°C. For non-denaturing conditions, incubate overnight.

DeGlycoMx enzyme solution includes:
PNGase F
Sialidase
ß-Galactosidase
Glucosaminidase
O-Glycosidase

This kit also includes buffer, denaturant solution, and Triton-X.

View product documentation as PDF

Price: $304.00

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Enzymatic CarboRelease Kit

KE-DG01

Kit includes the enzymes and buffers required to remove all N-linked oligosaccharides and many O-linked sugars from 2 mg of glycoprotein, via twenty 100 microgram samples.

The enzymes are packaged separately in individual 20 microlitre vials. This gives researchers the flexibility to characterize the glycans attached to their glycoprotein more fully than with our mixture of enzymes. Appropriate use of individual glycosidases allow the determination of sialyation (use of Sialidase only) and the specific presence of N-linked glycans (using PNGase F only) and O-linked glycans (using Sialidase, ß-Galactosidase, O-Glycosidase, and Glucosaminidase).

The kit is easy-to-use and effective: add 1 µl of each enzyme and incubate for 3 hours at 37°C.

Includes:
PNGase F
Sialidase
ß-Galactosidase
Glucosaminidase
O-Glycosidase.

This kit also includes buffer, denaturant solution, Triton X, and fetuin glycoprotein standard.

View product documentation as PDF

Price: $546.00

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Orela O-linked Glycan Release Kit

The Ludger Liberate™ Orela Kit has been developed for the release of O-linked glycans from glycoproteins.

Designed for easy, routine analysis of O-glycosylation by QC labs.
Straightforward and simple method.
Released glycans have free reducing terminii suitable for fluorescent labelling prior to analysis by HPLC and MS.
Fluorescent labelling allows relative quantitation of glycan species by HPLC.
Faster and lower cost than hydrazinolysis.
No special handling techniques.

The kit contains reagents for the release of O-linked glycans from glycoprotein biopharmaceuticals. Released glycans have free reducing terminii to allow fluorescent tagging by reductive amination. It includes reagents and materials for up to 12 glycoprotein samples analysed in parallel or two sets of 6 samples, typically up to 1 mg of glycoprotein per sample.

View product documentation as PDF

Part number: LL-ORELA-A2

Price: $799.00

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Endo F Multi-Kit

Part number KE-EFX3

Kit Contents
20 µl of Endo F1
20 µl of Endo F2
20 µl of Endo F3

Applications

Deglycosylation of native proteins resistant to PNGAse F cleavage
Determination of glycan type (high mannose, biantennary, tri/tetrantennary)
Deglycosylating proteins which normally precipitate when deglycosylating
X-Ray Crystallography

View product documentation as pdf file

Price: $385.00

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PNGase F

PNGase F - Elizabethkingia miricola (was Chryseobacterium meningosepticum)

E-PNG01
0.3 Unit / 60 uL
Includes enzyme, buffer, denaturant, and Triton-X.

View product documentation as PDF

Price: $385.00

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PNGase F - recombinant

PNGase F recombinant- Elizabethkingia miricola (was Chryseobacterium meningosepticum)

E-rPNG01
0.3 Unit / 60 uL
Includes enzyme, buffer, denaturant, and Triton-X.

View product documentation as PDF

Price: $385.00