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Enzymatic DeGlycoMx Kit
Enzymatic CarboRelease Kit
Orela O-linked Glycan Release Kit
PNGase F
PNGase F - recombinant
Endoglycosidases
Exoglycosidases
Sialic Acid Products
Purification Cartridges and Plates
Glycan Labeling Kits
HPLC Columns and Buffers
Glycan Standards
CarboSequencing
MS/MS Data Analysis Tools

Endo F Multi-Kit

The Endo F family of enzymes cleave asparagine-linked oligosaccharides They cleave between the two N-acetylglucosamine residues in the core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. The remaining monosacharide imparts a charge, thereby increasing the solubility of the protein. In contrast, PNGase F removes the oligosaccharide intact.

multiF
Part number KE-EFX3

Kit Contents
20 µl of Endo F1
20 µl of Endo F2
20 µl of Endo F3

Applications
  • Deglycosylation of native proteins resistant to PNGAse F cleavage
  • Determination of glycan type (high mannose, biantennary, tri/tetrantennary)
  • Deglycosylating proteins which normally precipitate when deglycosylating
  • X-Ray Crystallography
View product documentation as pdf file

 
Price: $385.00