| O-linked oligosaccharides are released from the glycoprotein chemically using alkaline degradationUnlabeled oligosaccharides are separated by anion exchange chromatographyGlycans are separated based on negative charge. This assay is specific for oligosaccharides linked to the amino acids serine or threonine in proteins, referred to as O‑linked oligosaccharides or O-glycans.   |