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Orela O-linked Glycan Release Kit
The Orela reaction:
- Liberation of the glycans as the glycosylamine derivative
The Orela reagent reacts at the link between the glycan and peptide backbone to liberate glycans as the
glycosylamine.
- Conversion of the glycosylamine to the free glycans
The acid-labile glycosylamine derivative is hydrolysed to produce the free glycan
Orela Kit Components
| Part # |
Item |
Quantity |
| LL-ORELAREAGENT-0 |
Orela Release Reagent |
2 x 3ml |
| LL-ACETIC-50P-01 |
Acetic Acid Solution |
2 x 3ml |
| LC-CEX-H-01 |
LudgerClean CEX-H cartridges |
2 x 6 cartridges |
| LL-COLLV-01 |
Collection vials with PTFE lined caps |
2 x 6 vials |
| LL-REACT-01 |
Reaction vials with PTFE lined caps |
2 x 6 vials |
Outline of the Orela Protocol
- Prepare the glycoconjugate
Prepare the glycoprotein or glycopeptide samples by removing contaminants such as salts, detergents and dyes that could interfere with the labeling procedure.
- Dry the glycans
Place the samples in reaction vials and dry down.
- Add release reagent to glycoconjugates
Add Orela release reagent to each sample.
- Incubate
Incubate the samples to allow the release reaction to progress.
- Acidification
Add acetic acid solution and incubate at 4C to convert glycosylamines into non-reduced glycans
- Post-release cleanup
Remove the peptide or protein using a cation exchange column
- Store or Analyse released glycans
The released glycans are now ready for analysis
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