PBM Glycosidase Cleanup Plate
For removal of exoglycosidase enzymes after glycan sequencing. This will prevent
contamination of HPLC columns during subsequent chromatographic analysis. The
plate can also be used to remove exoglycosidases or other proteins before mass
spectrometry analysis of glycans.
For removal of PNGase F and other protein and peptide material following the
acidification of N-glycans following their release from glycoproteins. Using this protein
binding membrane will provide cleaner samples and increase the signal for the
subsequent fluorescent labelling of the N-glycans. The plate can also be used to
remove PNGase F and other protein and peptide material before permethylation and/or
mass spectrometry analysis of N-glycans.
Description The LC-PBM-96 plate is a 96 well membrane-bottom plate containing a specialized
protein binding membrane with a nominal pore size of 0.45 µm. This product is
designed for use with both the vacuum manifold that can be purchased from
Ludger or with other popular vacuum manifold systems. Glycans pass through the
membrane whilst proteins are bound to the membrane allowing separation of these two
Number of Samples Sufficient for up to 96 samples.
Amount of Sample Up to 350 µL per well
Suitable Samples Unlabelled glycans from glycoproteins, fluorophore labelled glycans e.g. 2-AB or 2-AA
from glycoproteins, released and acidified N-glycans from glycoproteins
Storage Store at room temperature. Protect from sources of heat, light, and moisture. When
stored correctly, the reagents should be stable for 36 months from date of purchase.
Shipping The product should be shipped at ambient temperature.