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Deglycosylation kits
Endoglycosidases
Exoglycosidases
Sialic Acid Products
DMB Sialic Acid Labeling Kit
uR2 2.1x100mm UHPLC Column for Sialic Acid Analysis
SialiQuant Sialic Acid Quantitation Kit
Sialidase Au
Alpha-(2-3,6,8,9)
Sialidase Cp
Alpha-(2-3,6)
Sialidase Sp
Alpha-(2-3)
Sialic Acid Aldolase
CMP-Sialic Acid Synthetase
Purification Cartridges and Plates
Labeling Kits
HPLC Columns and Buffers
Standards
Sialic Acid Products
Sialic Acids are most often found at the terminal position of glycans, and therefore are a likely point of contact for glycan-ligand interactions.

Biopharmaceutical sialylation refers to the type and distribution of sialic acids in the glycans of therapeutic glycoproteins. Sialylation can significantly influence the safety and efficacy profiles of these drugs. In particular, the in vivo half-life of some biopharmaceuticals correlates with the degree of oligosaccharide sialylation.

Furthermore, the sialylation pattern can be a very useful measure of product consistency during manufacturing. Advances in our understanding of these issues have led the FDA, EMEA and other regulators to tighten their rules on glycoprofiling throughout the drug life cycle.

The two main types of sialyl residues found in biopharmaceuticals produced in mammalian expression systems are N-acetyl-neuraminic acid (NeuAc) and N-glycolylneuraminic acid (NeuGc). These usually occur as terminal structures attached to galactose (Gal) residues at the non-reducing terminii of both N- and O-linked glycans.

Controlling the ratio of NeuAc to NeuGc is critical for biomanufacturers. The reason is that NeuAc is the desired, normal human-type sialylation, while NeuGc is found in non-human glycoproteins and is considered an undesired, aberrant form of sialylation for therapeutic glycoproteins.

Read more from Biopharmaceutical Sialylation

DMB.png DMB Sialic Acid Labeling Kit

The LudgerTag DMB Labeling Kit has been developed for the qualitative analysis of sialic acids

The kit contains two sets of the labeling reagents and a sialic acid reference panel (pictured):
- Sialic Acid Reference Panel
- DMB Dye (1,2-diamino-4,5-methylenedioxybenzene.2HCl)
- Acetic Acid
- Mercaptoethanol in Acetic acid
- Sodium Dithionite (Reductant)

Typically, up to 15 separate analytical samples can be labeled per set.

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-KDMB-A1

Price: $691.00
 
UR2x100.png uR2 2.1x100mm UHPLC Column for Sialic Acid Analysis

Developed for the analysis of sialic acids labeled with 1,2-diamino-4,5 methylenedioxybenzene.2HCl (DMB) using UHPLC. The LudgerSep uR2 UHPLC column contains particles with an endcapped octadecylsilane coating optimized for hydrophobic chromatography.

View product documentation as PDF

Dimensions: 2.1mm x 100mm

View Certificate of Analysis as PDF

Part Number: LS-UR2-2.1x100

Price: $1,625.00
 
Sialiquant.png SialiQuant Sialic Acid Quantitation Kit

The SialiQuantâ„¢ Sialic Acid Quantitation Kit contains all the reagents to quickly and accurately quantitate sialic acids, including N-acetylneuraminic acid (NANA), N-glycolylneuraminic acid (NGNA). This kit includes reagents for 25 assays, each assay measuring from 1-200 nmoles of sialic acid.

In this method, N-Acetylneuraminic acid aldolase catalyzies the reversible reaction:
N-Acetylneuraminic acid N-Acetylmannosamine + Pyruvic acid

The pyruvic acid can be reduced to lactic acid by -NADH and lactic dehydrogenase:
Pyruvic Acid + -NADH Lactic Acid + -NAD

Under the proper conditions, the first forward reaction predominates, and when coupled with -NADH reduction of pyruvic acid, the reaction goes to completion. -NADH oxidation can be accurately measured spectrophotometrically.

View product documentation as PDF

Part Number: KE-SIALIQ

Price: $528.00
 
E-S001 drawing.gif Sialidase Au
Alpha-(2-3,6,8,9)

recombinant from A. ureafaciens

View product documentation as PDF

Part Number: E-S001

Price: $446.00
 
E-S005 drawing Sialidase Cp
Alpha-(2-3,6)

recombinant from C. perfringens

View product documentation as PDF

Part Number: E-S005

Price: $446.00
 
E-S007 drawing Sialidase Sp
Alpha-(2-3)

recombinant from Streptococcus pneumoniae in E. Coli

View product documentation as PDF

Part Number: E-S007

Price: $446.00
 
E-ALD01 Sialic Acid Aldolase

Recombinant from E. Coli
Converts sialic acid to N-acetylmannosamine & pyruvate Sialic acids can then be quantitated by a coupled reaction with NADH and lactic dehydrogenase

View product documentation as PDF

Part Number: E-ALD01

Price: $446.00
 
E-CMP-SAS drawing.gif CMP-Sialic Acid Synthetase

recombinant from E. Coli
This enzyme catalyzes the synthesis of CMP sialic acid from CTP & sialic acid.
The resulting molecule can then be covalently attached to an oligosaccharide by sialic acid transferase.

View product documentation as PDF

Part Number: E-CMP01

Price: $446.00
 
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