V-Tag Glycopeptide Labeling Kit
Application: For the fluorophore labeling and enrichment of glycopeptides.
Description: This kit contains the reagents needed for the conjugation of V-tag dye to
the amine moieties of glycopeptides, and the specialised solid phase extraction (SPE)
cartridges required for the purification and enrichment of the labeled glycopeptides.
Mass = 434.42 (resulting in a glycopeptide mass increase of 319.33 Da.)
Fluorescence, λex = 250 nm, λem = 360 nm.
Number of Samples: 24 separate analytical samples per kit.
Amount of Sample: From approximately 1 µg to 50 µg of glycoprotein per sample.
Suitable Samples: IgG single subclass glycoproteins. Other glycoproteins can be used, but note that if
there are multiple glycosylation sites, the glycopeptides may need to be separated by
C18 HPLC before HILIC analysis. It is recommended that samples do not have amine
containing solvents (e.g. Tris type buffers) or reduction/alkylation agents as they will
interfere with the V-tag labeling. Removal of the amine containing solvents and
reduction/alkylation agents can be accomplished using devices which are designed for
desalting and buffer exchange, as well as removal of low-molecular weight compounds
Labeling Selectivity: One V-tag label for every IgG glycopeptide N-terminus.
Storage: Store the V-tag dye at -20°C in the dark. Protect from sources of heat, light and
moisture. Once used, extra dye solution can be frozen and re-used.
Shipping: The product can be shipped at ambient temperature.