Neuraminidase, N-acetylneuraminate glycohydrolase
Part number E-S005
Source recombinant from Clostridium perfringens in E. Coli.
60 µl aliquot of enzyme (900 mU) in 20 mM Tris-HCl, 25 mM NaCl, pH 7.5
5x Reaction Buffer 250 mM sodium phosphate, pH 6.0
Specific Activity >250 U/mg
Activity 15 U/ml
Molecular weight~41,000 daltons
pH range 4.5-7, optimum 6.0
1. Add up to 100 µg of glycoprotein or 1 nmole of oligosaccharide to a tube.
2. Adjust to 14 µl final volume with de-ionized water.
3. Add 4 µl 5x reaction buffer (pH 6.0)
3. Add 2 µl of Sialadase Cp
4. Incubate 1 hour at 37°C.
Specifictity Cleaves all nonreducing terminal non-branched alpha-(2-3) and alpha-(2-6)-sialic acid residues from complex carbohydrates and glycoproteins.
Relative cleavage rates for different linkages are: (2-3) > (2-6).
Specific Activity Defined as the amount of enzyme required to produce 1 µmole of methylumbelliferone in 1 minute at 37°C, pH 5.0 from MU-NANA [2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid].
Storage Store enzyme at 4°C.
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Kobata, A. Use of endo- and exoglycosidases for structural studies of glycoconjugates. Anal Biochem 100: 1-14 (1979).
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Roggentin P., R.G . Kleineidam and R. Schauer. Diversity in the properties of two sialidase isoenzymes produced by Clostridium perfringens spp. Biol Chem Hoppe-Seyler 376: 569-575 (1995)