ß-(1-4)-Galactosidase

Part number E-BG07
Source recombinant Streptococcus pneumoniae
EC 3.2.1.23

Contents
60 µl aliquot of enzyme (180 mU) in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).
200 µl 5x Reaction Buffer 6.0 (250 mM sodium phosphate, pH 6.0).

Specific Activity >6 U/mg
Activity 3 U/ml

Molecular weight ~250,000 daltons

Suggested Usage Add 2 µL of enzyme to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide. Add buffer and incubate 1 hour at 37 °C.

Specifictity Non-reducing terminal ß-(1-4)-galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.

Specific Activity One unit of ß-(1-4)-galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C pH 5 from p-nitrophenyl-beta-D-galactopyranoside.

Storage Store enzyme at 4°C.