Part number LZ-CER-HM-KIT
Source Hirudo medicinalis
Ceramide Glycanase (in Sodium Acetate, Sodium
Taurodeoxycholate Hydrate and Bovine Serum Albumin)
Reaction Buffer (Sodium Acetate and Sodium Taurodeoxycholate
Add 5 µL of buffer to 5 µL of approximately 1 nmol of the glycolipid sample. For the positive control reaction, add 5 µL of GLIP-GM1-01 substrate to 5 µL of LZ-CER-BUFFX4 into an Eppendorf tube. Add 5 µL of enzyme. Make total reaction volume 20 µLs with the addition of water and incubate 24 hours at 37°C.
Specifictity Ceramide glycanase is an enzyme used to release glycans from GSLs to enable their characterisation. It cleaves glycans including GM1, GM2 and GM3 by cleaving the β-glycosyl linkage.
Storage Store enzyme at 4°C.