O-Linked Labeling Cleanup - CEX Cartridges
Application For purification of O-glycans from non-carbohydrate material including salts, proteins, and detergents. Applications include cleanup of glycans following hydrazinolysis or any other non-reductive O-glycan release method.
Description The cartridges contain a cation exchange resin that is stored in 1 M hydrochloric acid.
Binding capacity Each LudgerClean™ CEX cartridge can typically clean up O-glycans released from 1000 µg of glycoprotein.
Number of Samples LudgerClean™ CEX cartridges are designed for single use only.
Suitable Samples A wide range of O-glycans can be purified including di-saccharides, tri-saccharides and oligosaccharides. Glycan samples must be applied to the cartridges in solutions that are substantially aqueous.
Structural Integrity No detectable loss of sialylated glycans.
Binding Selectivity Essentially stoichiometric binding and elution for most complex glycan mixtures.
Storage Store refrigerated at 4 to 10°C in the dark. Protect from sources of heat, light, and moisture. The cartridges are stable for at least 6 months from date of manufacture.