Part number E-EF01
Endo F1, Endoglycosidase F1, endo-beta-N-acetylglucosaminidase F
Source recombinant Elizabethkingia miricola (was Chryseobacterium meningosepticum) in E. Coli
60 µl aliquot of enzyme (1 U) in 20 mM Tris-HCl, pH 7.5
5x Reaction Buffer - 250 mM sodium phosphate, pH 5.5
Specific Activity >16 U/mg
Activity 17 U/ml
Molecular weight 32,000 daltons
1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µl final volume with de-ionized water.
2. Add 10 µl 5x Reaction Buffer 5.5
3. Add 2.0 µl of Endo F1. Incubate 1 hour at 37°C.
Cleaves all asparagine-linked hybrid or high mannose oligosaccharides
Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of denatured Ribonuclease B (RNase B) in 1 minute at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE (cleaved RNase B migrates faster).
Storage Store enzyme at 4°C.