Endo F2

Part number E-EF02
Endo F2, Endoglycosidase F2, endo-beta-N-acetylglucosaminidase F2

Source recombinant Elizabethkingia miricola (was Chryseobacterium meningosepticum)in E. Coli

EC 3.2.1.96

Contents
60 µl aliquot of enzyme (0.3 U) in 10 mM sodium acetate 25mM NaCl, pH 4.5
5x Reaction Buffer - 250 mM sodium acetate, pH 4.5

Specific Activity >20 U/mg
Activity 5 U/ml

Molecular weight 32,000 daltons

Suggested usage
1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µl final volume with de-ionized water.
2. Add 10 µl 5x Reaction Buffer 4.5
3. Add 2.0 µl of Endo F2. Incubate 1 hour at 37°C.

Specifictity
Cleaves all asparagine-linked biantennary oligosaccharides and high mannose (though at a 40X reduced rate) N-glycans from peptides and proteins

Specific Activity
Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of denatured Ribonuclease B (RNase B) in 1 minute at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE (cleaved RNase B migrates faster).

Storage Store enzyme at 4°C.