Part number E-EF03
Endo F3, Endoglycosidase F3, endo-beta-N-acetylglucosaminidase F
Source recombinant Elizabethkingia meningosepticum (was Chryseobacterium meningosepticum)
60 µl aliquot of enzyme (0.33 U) in 20 mM Tris-HCl, pH 7.5
5x Reaction Buffer - 250 mM sodium acetate, pH 4.5
Specific Activity >25 U/mg
Activity 5 U/ml
Molecular weight 30,000 daltons
1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µl final volume with de-ionized water.
2. Add 10 µl 5x Reaction Buffer 4.5
4. Add 2.0 µl of Endo F3. Incubate 1 hour at 37°C.
Cleaves all asparagine-linked triantennary or fucosylated biantennary oligosaccharides, as well as triamannosyl chitobiose core structures. Alpha 1-3 fucosylation will inhibit enzymatic activity.
The recombinant version is not glycosylated, which may result in properties differing from the native protein.
Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of porcine fibrinogen in 1 minute at 37°C, pH 4.5. Cleavage is monitored by SDS-PAGE (cleaved fibrinogen migrates faster).
Storage Store enzyme at 4°C.