Part number E-AM01
Source Jack Bean
60 µl aliquot of enzyme (0.6 U) in 150 mM sodium phosphate (pH 7.5).
200 µl 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0).
Specific Activity 3 U/mg
Activity 10 U/ml
Molecular weight two polypeptides of64,000 and 44,000 daltons
Add 1 µL of enzyme to 1 nmol of oligosaccharide.
Add water to 15 µl
Add 4 µl 5x Reaction Buffer and incubate one hour at 37 °C.
Specificty All Alpha-(1-2,3,6)-linked mannose
One unit of Alpha-(1-2,3,6)-Mannosidase is defined as the amount of enzyme required to hydrolyze 1 µmole of p-nitrophenyl-alpha-p-mannoside to p-nitrophenol in 1 minute at pH 5.0 and 37°C.
Storage Store enzyme at 4°C.