Alpha-(1-3,6)-Galactosidase

Part number E-AG01
Source rec. from E.Coli
EC 3.2.1.22

Contents
60 µl aliquot of enzyme (24 U) in 50 mM sodium phosphate, pH 7.5
1 vial reaction buffer- 250mM Sodium phosphate, pH 6.5

Specific Activity >30 U/mg
Activity 400 U/ml

Molecular weight ~80,000 daltons

Suggested usage Add 2 µL of enzyme to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide. Add buffer and incubate one hour at 37°C.

Specificity Cleaves Alpha-(1-3)-and Alpha-(1-6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.

Specific Activity One unit of Alpha-(1-3,6)-Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 25°C pH 6.5 from p-nitrophenyl-alpha-D-galactopyranoside.