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2-AA Labeling kit
Part number LT-KAA-A2 Applications
Oligosaccharide profiling by:Mass Spectrometry [Harvey (2000), Harvey et al (2002), Huang et al (2001), Kuster et al (1996), Sato et al (2000)]HPLC [Anumula and Dhume (1998), Anumula and Du (1999), Bigge et al (1995)]Gel electrophoresis [Bigge et al (1995), Frears et al (1999), Huang et al (2000)]
Monosaccharide composition analysis by:
HPLC (C18 reverse phase) [Anumula (1994)]Capillary electrophoresis [Sato et al (1997)]Notes
2-AA is a superior replacement for 2-AB (2-aminobenzamide) in most types of complex glycan analysis. 2-AA is reported to have a higher fluorescence and gives higher labelling efficiencies than 2-AB.
Please note that there are a number of different conditions described in the literature for glycan labelling with 2-AA. This kit uses the conditions used by Bigge et al (1995). Please contact us if you would like to use other labeling conditions.
Kit Specifications
One kit contains reagents to label up to 30 separate analytical samples per kit. Each kit contains two sets of the following reagents (15 samples per set) supplied in glass ampoules sealed under pure nitrogen:
- 2-aminobenzoic acid (2-AA dye)
- Dimethyl sulfoxide (DMSO)
- Acetic acid
- Sodium cyanoborohydride
Dye purity: > 99% by HPLC. Soluble in DMSO and ethanol.
Molecular weight : 137 dalton
Excitation wavelength: 320 nm
Emission wavelength: 420 nm.
References
Anumula, K.R. (1994)
Quantitative determination of monosaccharides in glycoproteins by high-performance liquid chromatography with highly sensitive fluorescence detection.
Analytical Biochemistry 220:275-283
Anumula, KR; Dhume, ST (1998)
High resolution and high sensitivity methods for oligosaccharide mapping and characterization by normal phase high performance liquid chromatography following derivatization with highly fluorescent anthranilic acid.
Glycobiology 8:685-694
Anumula, KR; Du, P (1999)
Characterization of carbohydrates using highly fluorescent 2-aminobenzoic acid tag following gel electrophoresis of glycoproteins.
Analytical Biochemistry 275:236-242
Bigge, JC; Patel, TP; Bruce, JA; Goulding, PN; Charles, SM; Parekh, RB (1995)
Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid.
Analytical Biochemistry 230:229-238
Frears, ER; Merry, AH; Axford, JS (1999)
Screening neutral and acidic IgG N-glycans by high density electrophoresis.
Glycoconjugate Journal 16:283-290
Huang, Z; Prickett, T; Potts, M; Helm, RF (2000)
The use of the 2-aminobenzoic acid tag for oligosaccharide gel electrophoresis.
Carbohydrate Research 328:77-83
Sato, K; Sato, K; Okubo, A; Yamazaki, S (1997)
Determination of monosaccharides derivatized with 2-aminobenzoic acid by capillary electrophoresis.
Analytical Biochemistry 251:119-121
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