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2-AB Labeling kit
Part number: LT-KAB-A2 View product documentation as PDF View protocol picture guide as a PDF Applications
Oligosaccharide profiling by HPLC
- General methods papers [Bigge et al (1995), Davies and Hounsell (1996), Guile et al (1996), Hardy (1997, Kotani and Takasaki (1998), Rudd et al (2001), Townsend et al (1996)]
- Recombinant glycoproteins [Gohlke et al (2000), Hills et al (2001), Inoue et al (1999), James et al (1996), Kanazawa et al (1999), Klausen et al (1998), Manya et al (2000), Routier et al (1997), Sato et al (1999), Viseux et al (2001), Wuttke et al (2001)]
- Natural and recombinant antibodies [Routier et al (1997, 1998)]
- O-glycans [Chiba et al (1997), Merry et al (2002), Sasaki et al (1998), Tran et al (1998, 2001)]
Oligosaccharide profiling by MS
- [Harvey (2000), Harvey et al (2002), Huang et al (2001), Kuster et al (1996), Sato et al (2000)]
Notes
2-AB (2-aminobenzamide) is one of the most widely used fluorescent labels for glycosylation analysis.
Please note that there are a number of different conditions described in the literature for glycan labelling with 2-AB. This kit uses the conditions used by Bigge et al (1995). Please contact us if you would like to use other labeling conditions.
Kit Specifications
The kit contains two sets of the following reagents (15 samples per kit) supplied in glass ampoules sealed under pure nitrogen:
- 2-aminobenzamide (2-AB dye)
- Dimethyl sulfoxide (DMSO)
- Acetic acid
- Sodium cyanoborohydride
One kit contains reagents to label up to 30 separate analytical samples per kit.
Dye purity: > 99% by HPLC. Soluble in DMSO and ethanol. M.W. 137. lambda-ex = 320 nm, lambda-em = 420 nm.
References
- Anumula KR, Dhume ST. (1998)
High resolution and high sensitivity methods for oligosaccharide mapping and characterization by normal phase high performance liquid chromatography following derivatization with highly fluorescent anthranilic acid.
Glycobiology 8: 685-694
- Bigge JC, Patel TP, Bruce JA, Goulding PN, Charles SM, Parekh RB. (1995)
Nonselective and efficient fluorescent labeling of glycans using 2- amino benzamide and anthranilic acid.
Anal Biochem 230: 229-238
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