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| Sialic Acid Analysis
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This assay measures N-acetylneuraminic acid (NeuAc/NANA) and N-glycolylneuraminic acid (NeuGc/NGNA), the two most common sialic acids on recombinant glycoproteins. These monosaccharides cannot be measured using the same assay as the neutral monosaccharides since they are more easily destroyed by acid, therefore require a milder acid hydrolysis.
Sialic acids are released from glycoproteins, oligosaccharides, polysaccharides or other glycans using a mild acid hydrolysis. NeuAc and NeuGc are then separated by HPLC (high performance liquid chromatography). The amount of each monosaccharide in the sample can be measured by comparing the response in the sample to a standard curve of each sialic acid.
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