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| New Products
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QA-Bio and our partners continually develop new products
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| Deglycosylation kits
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QA-Bio provides easy to use kits for deglycosylating proteins in both native & denatured conditions.
PNGase F is commonly used to deglycosylate glycoproteins containing only N-linked glycans, working in both denatured and native conditions. For native samples that are slowly cleaved by PNGase F or those that precipitate after PNGase F digestion, our Endo F Multi-Kit is recommended. Both methods leave the glycans intact and available for further analysis.
If the glycan type is unknown, or if it consists of both N-linked & O-linked glycans, the use of our Enzymatic CarboRelease Kit or our Enzymatic DeGlycoMx Kit is recommended. These kits include a number of enzymes which will remove and digest the glycans into monosaccharides. In the DeglycoMx Kit, the enzymes are packaged as a premixed cocktail of enzymes in one 20 microlitre vial. For the CarboRelease Kit, the enzymes are supplied separately in individual vials of 20 microlitres, allowing greater flexibility in assay design.
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| Endoglycosidases
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- Cleave intact glycans from glycoprotein
- Highly stable enzymes in clean preparations- no glycerol, NaCl, or other additive.
- All enzymes are tested for the absence of proteolytic or unexpected glycosidic activity
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| Exoglycosidases
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- Cleave specific terminal-monosacharides from glycans
- Allows detailed analysis of glycan structure
- All enzymes are tested for the absence of proteolytic or unexpected glycosidic activity
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| Sialic Acid Products
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| Sialic Acids are most often found at the terminal position of glycans, and therefore are a likely point of contact for glycan-ligand interactions. Biopharmaceutical sialylation refers to the type and distribution of sialic acids in the glycans of therapeutic glycoproteins. Sialylation can significantly influence the safety and efficacy profiles of these drugs. In particular, the in vivo half-life of some biopharmaceuticals correlates with the degree of oligosaccharide sialylation.
Furthermore, the sialylation pattern can be a very useful measure of product consistency during manufacturing. Advances in our understanding of these issues have led the FDA, EMEA and other regulators to tighten their rules on glycoprofiling throughout the drug life cycle. The two main types of sialyl residues found in biopharmaceuticals produced in mammalian expression systems are N-acetyl-neuraminic acid (NeuAc) and N-glycolylneuraminic acid (NeuGc). These usually occur as terminal structures attached to galactose (Gal) residues at the non-reducing terminii of both N- and O-linked glycans. Controlling the ratio of NeuAc to NeuGc is critical for biomanufacturers. The reason is that NeuAc is the desired, normal human-type sialylation, while NeuGc is found in non-human glycoproteins and is considered an undesired, aberrant form of sialylation for therapeutic glycoproteins. Read more from Biopharmaceutical Sialylation |
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| Purification Cartridges
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- For the cleanup of glycans after enzyme digestions and fluorescent labeling
- Cartridges bind glycans but allow contaminants to be washed through
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| Glycan Labeling Kits
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Developed for:
- Quantitative glycoprofiling of biopharmaceuticals
- Improved sensitivity for ESI and MALDI-TOF mass spec analysis
- Modifying glycan fragmentation patterns during MSn analysis
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LudgerTag Glycan Labeling Kits
LudgerTag kit reagents are purified to analytical grade and are dispensed and sealed under clean, inert atmospheres. The ampoules are pre-cleaned by pyrolysis at 500°C then opened just before dispensing and sealing under oxygen-free dry nitrogen. These controls ensure that your analyses work properly each and every time.
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Acetic Acid
Promotes acid catalyzed ring opening of the glycan reducing terminus prior to the formation of an imine intermediate between the sugars and the unprotonated form of the dye.
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Fluorescent Dye
The comprehensive range of LudgerTag dyes include 2-AB, 2-AA, 2-AP, DMB, and AA-Ac to cover different types of glycoanalyses by HPLC and MS.
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DMSO
An anhdrous solvent used to dissolve all the components of the reductive amination labeling reaction.
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Sodium Cyanoborohydride
This reductant stabilizes the imine produced by the reaction of the dye and glycans to produce stable fluorescently labeled glycans.
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| HPLC Columns
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- Developed for quantitative glycoprofiling of biopharmaceuticals
- Individual tested and qualified for glycan analysis
- Reliable, Reproducible, Sensitive
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| Glycan Standards
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QA-Bio supplies both fluorescently labeled and unlabeled glycans.
All glycan standards are qualified to be >90% pure as assessed by a combination of H-NMR and HPLC.
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| CarboSequencing
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Includes the enzymes, and buffers required to sequence ten isolated, N-linked oligosaccharides.
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| MS/MS Data Analysis Tools
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 SimGlycan™ is an innovative MS/MS data analysis tool. SimGlycan™ predicts the glycan structure, scores it and generates a list of probable glycans that closely match the your experimental MS profile saving you the time and the frustration of laborious work. |
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