| The SialiQuant™ Sialic Acid Quantitation Kit contains all the reagents to quickly and accurately quantitate sialic acids, including N-acetylneuraminic acid (NANA), N-glycolylneuraminic acid (NGNA). This kit includes reagents for 25 assays, each assay measuring from 1-200 nmoles of sialic acid. In this method, N-Acetylneuraminic acid aldolase catalyzies the reversible reaction: N-Acetylneuraminic acid ↔ N-Acetylmannosamine + Pyruvic acidThe pyruvic acid can be reduced to lactic acid by β-NADH and lactic dehydrogenase: .
Pyruvic Acid + β-NADH ↔ Lactic Acid + β-NADUnder the proper conditions, the first forward reaction predominates, and when coupled with β-NADH reduction of pyruvic acid, the reaction goes to completion. β-NADH oxidation can be accurately measured spectrophotometrically. View product documentation as PDF Part number KE-SIALIQ |
