PNGase F is commonly used to deglycosylate glycoproteins containing only N-linked glycans, working in both denatured and native conditions. For native samples that are slowly cleaved by PNGase F or those that precipitate after PNGase F digestion, our Endo F Multi-Kit is recommended. Both methods leave the glycans intact and available for further analysis.

If the glycan type is unknown, or if it consists of both N-linked & O-linked glycans, the use of our Enzymatic CarboRelease Kit is recommended. This kit includes a number of enzymes which will remove and digest the glycans into monosaccharides.